Development of a multiplexed PCR detection method for Barley and Cereal yellow dwarf viruses, Wheat spindle streak virus, Wheat streak mosaic virus and Soil-borne wheat mosaic virus.

Simultaneous detection of wheat dwarf virus, northern cereal mosaic virus, barley yellow striate mosaic virus and rice black-streaked dwarf virus in wheat by multiplex RT-PCR.

Wheat dwarf virus (WDV), barley yellow striate mosaic virus (BYSMV), rice black-streaked dwarf virus (RBSDV) and northern cereal mosaic virus (NCMV) are four viruses infecting wheat and causing similar symptoms. In this paper, a multiplex reverse transcription polymerase chain reaction (m-RT-PCR) method has been developed for the simultaneous detection and discrimination of these viruses. The p...

Reactions of Perennial Grain Accessions to Four Major Cereal Pathogens of the Great Plains

Problems associated with annual grain agriculture, such as soil erosion and water and air pollution, indicate that reliance on annual grain production is a fundamental problem of current agricultural practice (23). Annual plant roots are less efficient micromanagers of water, soil, and soil nutrients than their perennial counterparts, resulting in nutrient and pesticide contamination of water s...

Differential detection of Wheat yellow mosaic virus, Japanese soil-borne wheat mosaic virus and Chinese wheat mosaic virus by reverse transcription loop-mediated isothermal amplification reaction.

A differential detection method for three wheat viruses: Wheat yellow mosaic virus (WYMV), Japanese soil-borne mosaic virus (JSBWMV) and Chinese wheat mosaic virus (CWMV) using reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction was developed. All three primer sets, which were designed from the genome sequences of WYMV, JSBWMV and CWMV respectively, worked most effic...

Identification of wheat streak mosaic virus in iran

The effects of postplanting environment on the incidence of soilborne viral diseases in winter cereals.

ABSTRACT Soilborne wheat mosaic virus (SBWMV) and Wheat spindle streak mosaic virus (WSSMV) are putatively transmitted to small grains by the obligate parasite Polymyxa graminis, but little is known about environmental requirements for transmission and the resulting disease incidence. We planted susceptible wheat and triticale cultivars in field nurseries on different autumn dates in 3 years an...